TABLE 5

S. pombe php5 is important for fbp1 transcription

β-Galactosidase activity
StrainRelevant genotypeRepressedDerepressed
FWP112Wild type9 ± 11586 ± 83
RJP18php5Δ3 ± 0690 ± 95
RJP12tup11Δ tup12Δ956 ± 531780 ± 95
RJP66tup11Δ tup12Δ php5Δ110 ± 5483 ± 18
RJP72pka1Δ1864 ± 114ND
RJP31pka1Δ php5Δ1264 ± 83ND
RJP67atf1Δ8 ± 0147 ± 20
RJP39atf1Δ php5Δ1 ± 011 ± 0
  • β-Galactosidase activity was determined from four to six independent cultures as described in materials and methods. The average ± SE represents specific activity per milligram of soluble protein. ND, not determined.