TABLE 4

Genetic interactions between the tup12Δ tup11Δ double deletion and mutations in the MAPK or PKA pathways

β-Galactosidase activity
StrainRelevant genotypeRepressedDerepressed
FWP112Wild type9 ± 11586 ± 83
RJP12tup11Δ tup12Δ956 ± 531780 ± 95
RJP67atf1Δ8 ± 0147 ± 20
CHP720wis1Δ8 ± 079 ± 9
RJP36atf1Δ tup11Δ tup12Δ105 ± 11430 ± 28
RJP59pcr1Δ tup11Δ tup12Δ370 ± 20778 ± 147
RJP33wis1Δ tup11Δ tup12Δ572 ± 61140 ± 101
RJP41cgs1Δ tup11Δ tup12Δ72 ± 2279 ± 7
  • β-Galactosidase activity was determined from four to six independent cultures as described in materials and methods. The average ± SE represents specific activity per milligram of soluble protein.