TABLE 3

ptup12a partial suppression of a cgs1 deletion

β-Galactosidase activity
pLEV3 transformantptup12a transformant
StrainRelevant genotypeRepressedDerepressedRepressedDerepressed
FWP112Wild type10 ± 01095 ± 12615 ± 11218 ± 78
RJP12tup11Δ tup12Δ511 ± 302585 ± 263602 ± 362667 ± 325
RJP25cgs1Δ3 ± 090 ± 103 ± 1721 ± 233
CHP490pka1Δ3612 ± 622091 ± 804064 ± 1182730 ± 284
  • β-Galactosidase activity was determined from three to four independent cultures as described in materials and methods. The average ± SE represents specific activity per milligram of soluble protein.