TABLE 2

Effects of tup11Δ, tup12Δ, and scr1Δ deletions on fbp1-lacZ expression

β-Galactosidase activity
StrainRelevant genotypeRepressedDerepressed
FWP112Wild type9 ± 11586 ± 83
RJP8tup12Δ83 ± 111730 ± 73
RJP10tup11Δ40 ± 31127 ± 54
RJP12tup11Δ tup12Δ956 ± 531780 ± 95
RJP46scr1Δ138 ± 231886 ± 163
RJP63scr1Δ tup12Δ610 ± 1223023 ± 336
RJP48scr1Δ tup11Δ385 ± 591704 ± 129
RJP51scr1Δ tup11Δ tup12Δ743 ± 2582441 ± 157
  • β-Galactosidase activity was determined from six to eight independent cultures as described in materials and methods. The average ± SE represents specific activity per milligram of soluble protein.