TABLE 4

Sterol lipid levels after Osh1p-7p depletion

Wild-type cells
lipid amount
(μg/mg protein)
Osh1p-7p depleted cells
Sterol lipidLipid amount
(μg/mg protein)
Fold change relative
to wild type
Ergosterol67.8 ± 10.3237 ± 34.83.5
Zymosterol10.4 ± 2.731.0 ± 3.93.0
Episterola6.2 ± 3.48.1 ± 2.01.3
22-Dihydroergosterol5.6 ± 2.770.3 ± 14.713
Fungisterola4.1 ± 0.88.2 ± 2.02.0
Fecosterol4.0 ± 2.111.3 ± 1.42.9
Lanosterol3.8 ± 2.56.8 ± 0.41.8
  • a Identities of episterol and fungisterol were assigned solely on basis of molecular weight.