TABLE 4

Loss of a linear minichromosome in cbh2Δ and double mutant strains

StrainRelevant
genotype
Total colonies
assayed
Chromosome
loss per divisiona
Fold
increase
Spx34-10WT16,7195.7 (±1.8) × 10-4(1)
Spx34-6Acbh2Δ30,8197.8 (±3.1) × 10-314
Spx34-13cbh1Δ2,3578.9 × 10-316
SBP082996abp1Δ13,0493.2 (±0.33) × 10-36
Spx34-7Acbh1Δ cbh2Δ15,3011.2 (±0.49) × 10-221
Spx33-5Bcbh2Δ abp1Δ3,2563.9 (±0.97) × 10-268
Spx40-E9bmis6-30210,5364.9 × 10-39
Spx47-1bmis6-302 cbh2Δ4,3135.6 × 10-310
  • a Results are given as mean frequency of half-sectored colonies. Standard error is shown in parentheses for values derived from three or more independent platings.

  • b mis6-302 strains were tested at a low semipermissive temperature (23°).