TABLE 3

Sterol-related phenotypes of the OSH deletion allele combinations

Rich (+Trp)Rich MediumLovastatinNys (5 units)Nys (20 units)NaCl
Deletion genotypes23°30°37°23°30°37°23°30°37°30°37°30°37°23°30°37°
osh1Δ10S10S104S100S
osh2Δ100R
osh3Δ10R
osh4Δ1000R104R
osh5Δ10R10R
osh6Δ
osh7Δ10R
osh1Δosh2Δ10S10R
osh1Δosh3Δ10S10R10R
osh1Δosh4Δ1000S1000R100R100S100S10S
osh1Δosh5Δ104S10S
osh1Δosh6Δ10S10S10R10R
osh1Δosh7Δ100S10S10R100R10R10R
osh2Δosh3Δ
osh2Δosh4Δ10S100S10S1000S1000R1000R10S10S
osh2Δosh5Δ100R
osh2Δosh6Δ10R10R10R
osh2Δosh7Δ10R10R10R10R
osh3Δosh4Δ10S
osh3Δosh5Δ
osh3Δosh6Δ10R10S
osh3Δosh7Δ
osh4Δosh5Δ100R10R
osh4Δosh6Δ10S100R100R
osh4Δosh7Δ1000S10S100S1000R1000R
osh5Δosh6Δ10R10R
osh5Δosh7Δ100R1000R10R
osh6Δosh7Δ100R1000R100R100R10R
osh1Δosh2Δosh3Δ100S100S100S10S100S10S
osh1Δosh2Δosh4Δ100S10S1000S10S1000R100R10S10S100S
osh1Δosh2Δosh5Δ10R10R
osh1Δosh2Δosh6Δ10S10R10R
osh1Δosh2Δosh7Δ10S100R10R
osh1Δosh3Δosh4Δ10S10S100S1000S100S
osh1Δosh3Δosh5Δ
osh1Δosh3Δosh6Δ10S10R10R
osh1Δosh3Δosh7Δ100S
osh1Δosh4Δosh5Δ100S10S10S104S10S100S10R10R100S100S100S
osh1Δosh4Δosh6Δ100S100S1000S10S10R10S1000S
osh1Δosh4Δosh7Δ100S10S10S1000S10S1000S10S10S10S100R100S1000S1000S
osh1Δosh5Δosh6Δ10S100S
osh1Δosh5Δosh7Δ10S10R
osh1Δosh6Δosh7Δ10S10S100S10S10S100S10S10S104S10S1000S1000S
osh2Δosh3Δosh4Δ10S100S1000S100S10S100R100S1000S100S
osh2Δosh3Δosh5Δ
osh2Δosh3Δosh6Δ
osh2Δosh3Δosh7Δ10S1000S10R
osh2Δosh4Δosh5Δ10S1000R100R10S10S10S
osh2Δosh4Δosh6Δ10S10S1000R100R10S100S
osh2Δosh4Δosh7Δ10S10S10S104S10S104R10R104S
osh2Δosh5Δosh6Δ10R
osh2Δosh5Δosh7Δ10R10R
osh2Δosh6Δosh7Δ1000R104R10R10R
osh3Δosh4Δosh5Δ10S
osh3Δosh4Δosh6Δ100S
osh3Δosh4Δosh7Δ10S100S
osh3Δosh5Δosh6Δ
osh3Δosh5Δosh7Δ
osh3Δosh6Δosh7Δ100S10S10S10R10R10R
osh4Δosh5Δosh6Δ10S100S10S10S10S
osh4Δosh5Δosh7Δ10S1000R100R10R
osh4Δosh6Δosh7Δ104R104R10R10R
osh5Δosh6Δosh7Δ100R100R10R
osh1Δosh2Δosh3Δosh4Δ10S10S100S104S100S10R10R10S10S10S
osh1Δosh2Δosh3Δosh5Δ
osh1Δosh2Δosh3Δosh6Δ100S10S10S
osh1Δosh2Δosh3Δosh7Δ100S100S10S
osh1Δosh2Δosh4Δosh5Δ10S10S100S10S10S100S100S104S100S10R100S100S100S
osh1Δosh2Δosh4Δosh6Δ10S10S1000R1000R10S10S
osh1Δosh2Δosh4Δosh7Δ10S100S1000R100R10S
osh1Δosh2Δosh5Δosh6Δ10R
osh1Δosh2Δosh5Δosh7Δ10S100S10S
osh1Δosh2Δosh6Δosh7Δ10R10R10R10R
osh1Δosh3Δosh4Δosh5Δ10R10S10S10S
osh1Δosh3Δosh4Δosh6Δ10S1000S10S10S10S
osh1Δosh3Δosh4Δosh7Δ1000S1000S10S10S100S
osh1Δosh3Δosh5Δosh6Δ10S1000S10S10S
osh1Δosh3Δosh5Δosh7Δ10S10R10R
osh1Δosh3Δosh6Δosh7Δ
osh1Δosh4Δosh5Δosh6Δ100S100S100S100R100R10S10S10S
osh1Δosh4Δosh5Δosh7Δ10S100S10S10R10R10S10S100S
osh1Δosh4Δosh6Δosh7Δ100S100S104S100S1000S10R10R1000S105S1000S
osh1Δosh5Δosh6Δosh7Δ100S100S10R10R10R100R10R
osh2Δosh3Δosh4Δosh5Δ100S100S100S1000S100R100R10S100S10S
osh2Δosh3Δosh4Δosh6Δ1000S1000S100S100S10S10S10S100S
osh2Δosh3Δosh4Δosh7Δ100S10S1000S10S100S10S10S10S10S10R100R10S10S100S
osh2Δosh3Δosh5Δosh6Δ10S10R10R
osh2Δosh3Δosh5Δosh7Δ10S10S10S100S10S
osh2Δosh3Δosh6Δosh7Δ10S10S10R
osh2Δosh4Δosh5Δosh6Δ10S10S1000R1000R10S
osh2Δosh4Δosh5Δosh7Δ1000R1000R10S
osh2Δosh4Δosh6Δosh7Δ10S10S10S100S10S10S10S1000R100R
osh2Δosh5Δosh6Δosh7Δ10R10R10R10R
osh3Δosh4Δosh5Δosh6Δ10S10R
osh3Δosh4Δosh5Δosh7Δ1000S1000S10S10S
osh3Δosh4Δosh6Δosh7Δ10S100S10S10S10S10S
osh3Δosh5Δosh6Δosh7Δ10R
osh4Δosh5Δosh6Δosh7Δ100S1000S10R100R10R
osh1Δosh2Δosh3Δosh4Δosh5Δ10S10S10S10S100S1000S10S100R10R100S100S100S
osh1Δosh2Δosh3Δosh4Δosh6Δ10S10S10S10S10S1000S10S100S105S100S
osh1Δosh2Δosh3Δosh4Δosh7Δ*
osh1Δosh2Δosh3Δosh5Δosh6Δ10S1000S10S
osh1Δosh2Δosh3Δosh5Δosh7Δ10S10R
osh1Δosh2Δosh3Δosh6Δosh7Δ10S10S100S1000S10S100S10R10S
osh1Δosh2Δosh4Δosh5Δosh6Δ10S10S10S10S100S10R10R10S10S10S
osh1Δosh2Δosh4Δosh5Δosh7Δ105R1000R10R
osh1Δosh2Δosh4Δosh6Δosh7Δ*
osh1Δosh2Δosh5Δosh6Δosh7Δ100R100R10R10R10R
osh1Δosh3Δosh4Δosh5Δosh6Δ10S100S100S10S
osh1Δosh3Δosh4Δosh5Δosh7Δ100S1000S100S100S10S10S10S10S10S10S10S
osh1Δosh3Δosh4Δosh6Δosh7Δ10R10R10R10R
osh1Δosh3Δosh5Δosh6Δosh7Δ100S100S1000S10R10R10R
osh1Δosh4Δosh5Δosh6Δosh7Δ10S10S1000S100S10S100R100R10S10S10S
osh2Δosh3Δosh4Δosh5Δosh6Δ1000S10S1000S10S10S100S100S100S100S100S
osh2Δosh3Δosh4Δosh5Δosh7Δ1000S1000S1000S1000S100S1000S10S100S1000S104S1000S100S100S100S
osh2Δosh3Δosh4Δosh6Δosh7Δ10S100R10R10R10S
osh2Δosh3Δosh5Δosh6Δosh7Δ1000S10R
osh2Δosh4Δosh5Δosh6Δosh7Δ10S104R100R10R
osh3Δosh4Δosh5Δosh6Δosh7Δ100S1000S104S
osh1Δosh2Δosh3Δosh4Δosh5Δosh6Δ100S10S1000S100S100S100S1000S100S10S100S100S1000S
osh1Δosh2Δosh3Δosh4Δosh5Δosh7Δ10S10S100S1000S10S100S100S10S1000S100S1000S100S100S1000S
osh1Δosh2Δosh3Δosh4Δosh6Δosh7Δ*
osh1Δosh2Δosh3Δosh5Δosh6Δosh7Δ10S10S100S10R10R10R
osh1Δosh2Δosh4Δosh5Δosh6Δosh7Δ100S100S100S1000S100S100S100S100S100S100S100S100S100S100S
osh1Δosh3Δosh4Δosh5Δosh6Δosh7Δ100S1000S100S100S1000S104S10S10S10S
osh2Δosh3Δosh4Δosh5Δosh6Δosh7Δ
oshΔ
  • All viable OSH deletion combinations were tested for sterol-related phenotypes. The table quantitatively summarizes the defects observed on rich solid medium where 10-fold serial dilutions of each mutant strain and their wild-type parent (SEY6210) were spotted. In 10-fold increments, S denotes the magnitude of sensitivity to the conditions tested and R the resistance of the deletion mutants as compared to wild-type growth. Concentrations of additives (in rich medium) are as follows: 50 mg/liter tryptophan; 150 μg/ml lovastatin; 5 units/ml nystatin; 20 units/ml nystatin; 1.2 m NaCl; 0.7 m KCl; 1.0 m sorbitol; 0.15 m LiCl. Media containing nystatin, lovastatin, sorbitol, or any salt were also supplemented with 50 mg/liter tryptophan. At 23°, the wild-type strain grew poorly or not at all in the presence of 5 or 20 units/ml (unlike many of the deletion strains) and therefore was not useful for quantitative comparison. On rich medium, with or without tryptophan, and on sorbitol, 5 units/ml nystatin, LiCl, or KCl-containing medium, the strains were incubated for 1, 1–2, and 2 days at 37°, 30°, and 23°, respectively. All strains were incubated in the presence of 20 units/ml nystatin and lovastatin for 2, 3, and 4 days at 37°, 30°, and 23°, respectively. To determine relative resistance to 1.2 m NaCl, strains were examined after 2, 3, and 4 days at 37°, 30°, and 23°, respectively. To record sensitivities to 1.2 m NaCl, strains were examined after 3, 5, and 7 days at 37°, 30°, and 23°, respectively. To record 1.2 m NaCl resistance, all strains were examined after 2, 3, and 5 days at 37°, 30°, and 23°, respectively. Resistance to nystatin was recorded only as a function of high concentrations (20 units/ml nystatin), and sensitivity was recorded only for low concentrations (5 units/ml nystatin). Strains that grew on minimal medium but did not grow on rich medium are indicated with an asterisk (*). The last two strains listed represent lethal combinations of deletions.