TABLE 2

Abnormal morphology in cbh2Δ abp1Δ strains

Strain% branched or
multiseptated
% interphase% mitoticTotal no. of
cells scored
WT<0.52575202
cbh2Δ22672148
abp1Δ1.32673156
cbh2Δ abp1Δ121870184
  • Fixed, DAPI-stained cells from strains Sp223 (WT), Spx34-6A (cbh2Δ), SBP082996 (abp1Δ), and Spx33-5 (cbh2Δ abp1Δ) were examined by fluorescence microscopy. Morphologically normal cells exhibiting a clearly defined sickle-shaped nucleus characteristic of interphase were scored as interphase; all other normal cells were classed as mitotic. The percentage of cells in each category is given and the total number of cells scored is shown in the last column.