TABLE 1

Yeast strains used

StrainGenotypeOrigina
CBX2SEY6210 cyh2 [GRM1-96]b
CBX6SEY6210 cyh2 osh4Δ::HIS3 [GRM1-96, URA3]b
CBX7SEY6210 cyh2 osh7Δ::HIS3 [GRM1-96, URA3]b
CBX9SEY6210 cyh2 osh5Δ::LEU2 [GRM1-96, URA3]b
CBX10SEY6210 cyh2 osh6Δ::LEU2 [GRM1-96, URA3]b
CBX11SEY6210 cyh2 osh3Δ::LYS2 [GRM1-96, URA3]b
CBX12SEY6210 cyh2 osh2Δ::kan-MX4 [GRM1-96, URA3]b
CBX13MATα cyh2 ura3Δ::kan-MX4 his3Δ200 lys2-801 leu2-3,112 trp1Δ901 suc2Δ9 [GRM1-96, URA3]b
CBX14SEY6210 chy2 osh1Δ::kan-MX4 [GRM1-96, URA3]b
CBX20MATα chy2 ura3-52 lys2-801 leu2-3,112 trp1Δ901 suc2Δ9 [GRM1-96, URA3]b
CBX21MATα cyh2 ura3-52 his3Δ200 lys2-801 trp1Δ901 suc2Δ9 [GRM1-96, URA3]b
CBX22MATα cyh2 ura3-52 his3Δ200 leu2-3,112 trp1Δ901 suc2Δ9 [GRM1-96, URA3]b
CTY1-1AMATa ura3-52 his3Δ200 lys2-801 sec14-1Bankaitis et al. (1989)
HAB821SEY6210 osh4Δ/kes1Δ::HIS3Jiang et al. (1994)
HAB835SEY6210 osh1Δ::URA3Jiang et al. (1994)
JRY6200SEY6210 osh7Δ::HIS3
JRY6201SEY6210 osh6Δ::LEU2
JRY6202SEY6210 osh3Δ::LYS2
JRY6203SEY6210 osh2Δ::URA3
JRY6206SEY6210 osh5Δ/hes1Δ::LEU2
JRY6207SEY6210 osh6Δ::LEU2 osh7Δ::HIS3
JRY6208SEY6210 osh3Δ::LYS2 osh7Δ::HIS3
JRY6209SEY6210 osh2Δ::URA3 osh7Δ::HIS3
JRY6210SEY6210 osh1Δ::URA3 osh7Δ::HIS3
JRY6211SEY6210 osh4Δ::HIS3 osh7Δ::HIS3
JRY6212SEY6210 osh5Δ::LEU2 osh7Δ::HIS3
JRY6213SEY6210 osh3Δ::LYS2 osh6Δ::LEU2
JRY6214SEY6210 osh2Δ::URA3 osh6Δ::LEU2
JRY6215SEY6210 osh1Δ::URA3 osh6Δ::LEU2
JRY6216SEY6210 osh4Δ::HIS3 osh6Δ::LEU2
JRY6217SEY6210 osh5Δ::LEU2 osh6Δ::LEU2
JRY6218SEY6210 osh2Δ::URA3 osh3Δ::LYS2
JRY6219SEY6210 osh1Δ::URA3 osh3Δ::LYS2
JRY6220SEY6210 osh3Δ::LYS2 osh4ΔHIS3
JRY6221SEY6210 osh3Δ::LYS2 osh5Δ::LEU2
JRY6222SEY6210 osh1Δ::URA3 osh2Δ::URA3
JRY6223SEY6210 osh2Δ::URA3 osh4Δ::HIS3
JRY6224SEY6210 osh2Δ::URA3 osh5Δ::LEU2
JRY6225SEY6210 osh1Δ::URA3 osh4Δ::HIS3
JRY6226SEY6210 osh1Δ::URA3 osh5Δ::LEU2
JRY6227SEY6210 osh4Δ::HIS3 osh5Δ::LEU2
JRY6228SEY6210 osh3Δ::LYS2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6229SEY6210 osh2Δ::URA3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6230SEY6210 osh1Δ::URA3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6231SEY6210 osh4Δ::HIS3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6232SEY6210 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6233SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh7Δ::HIS3
JRY6234SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh7Δ::HIS3
JRY6235SEY6210c osh3Δ::LYS2 osh4Δ::HIS3 osh7Δ::HIS3
JRY6236SEY6210c osh3Δ::LYS2 osh5Δ::LEU2 osh7Δ::HIS3
JRY6237SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh7Δ::HIS3
JRY6238SEY6210 osh2Δ::URA3 osh4Δ::HIS3 osh7Δ::HIS3
JRY6239SEY6210 osh2Δ::URA3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6240SEY6210 osh1Δ::URA3 osh4Δ::HIS3 osh7Δ::HIS3
JRY6241SEY6210 osh1Δ::URA3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6242SEY6210 osh4Δ::HIS3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6243SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh6Δ::LEU2
JRY6244SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh6Δ::LEU2
JRY6245SEY6210 osh3Δ::LYS2 osh4Δ::HIS3 osh6Δ::LEU2
JRY6246SEY6210 osh3Δ::LYS2 osh5Δ::LEU2 osh6Δ::LEU2
JRY6247SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh6Δ::LEU2
JRY6248SEY6210 osh2Δ::URA3 osh4Δ::HIS3 osh6Δ::LEU2
JRY6249SEY6210 osh2Δ::URA3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6250SEY6210c osh1Δ::URA3 osh4Δ::HIS3 osh6Δ::LEU2
JRY6251SEY6210 osh1Δ::URA3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6252SEY6210 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6253SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2
JRY6254SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3
JRY6255SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2
JRY6256SEY6210c osh1Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3
JRY6257SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2
JRY6258SEY6210 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2
JRY6259SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh4Δ::HIS3
JRY6260SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh5Δ::LEU2
JRY6261SEY6210 osh2Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2
JRY6262SEY6210c osh1Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2
JRY6263SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6264SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6265SEY6210 osh3Δ::LYS2 osh4Δ::HIS3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6266SEY6210 osh3Δ::LYS2 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6267SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6268SEY6210c osh2Δ::URA3 osh4Δ::HIS3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6269SEY6210c osh2Δ::URA3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6270SEY6210c osh1Δ::URA3 osh4Δ::HIS3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6271SEY6210c osh1Δ::URA3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6272SEY6210 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6273SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh7Δ::HIS3
JRY6274SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh7Δ::HIS3
JRY6275SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2 osh7Δ::HIS3
JRY6276SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh7Δ::HIS3
JRY6277SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2 osh7Δ::HIS3
JRY6278SEY6210 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6279SEY6210c osh1Δ::URA3 osh2Δ::URA3 osh4Δ::HIS3 osh7Δ::HIS3
JRY6280SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6281SEY6210 osh2Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6282SEY6210 osh1Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6283SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh6Δ::LEU2
JRY6284SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh6Δ::LEU2
JRY6285SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2 osh6Δ::LEU2
JRY6286SEY6210c osh1Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh6Δ::LEU2
JRY6287SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2 osh6Δ::LEU2
JRY6288SEY6210 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6289SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh4Δ::HIS3 osh6Δ::LEU2
JRY6290SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6291SEY6210 osh2Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6292SEY6210 osh1Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6293SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3
JRY6294SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2
JRY6295SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2
JRY6296SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2
JRY6297SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2
JRY6298SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6299SEY6210c osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6300SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6301SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6302SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6303SEY6210c osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6304SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh4Δ::HIS3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6305SEY6210c osh1Δ::URA3 osh2Δ::URA3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6306SEY6210c osh2Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6307SEY6210c osh1Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6308SEY6210 osh1Δ::kan-MX4 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh7Δ::HIS3
JRY6309SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2 osh7Δ::HIS3
JRY6310SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6311SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6312SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6313SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh6Δ::LEU2
JRY6314SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2 osh6Δ::LEU2
JRY6315SEY6210 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6316SEY6210 osh1Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6317SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6318SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2
JRY6319SEY6210 osh1Δ::kan-MX4 osh2Δ::kan-MX4 osh3Δ::LYS osh4Δ::HIS3 osh6Δ::LEU2 osh7Δ::HIS3
JRY6320SEY6210c osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6321SEY6210 TRP1::PMET3-OSH2 osh2Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6322SEY6210c osh1Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6323SEY6210 osh1Δ::URA3 osh2Δ::kan-MX4 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
JRY6324SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh7Δ::HIS3
JRY6325SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2
JRY6326SEY6210 TRP1::PMET3-OSH2 osh1Δ::kan-MX4 osh3Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3
MS2339MATa ura3-52 leu2-3,112 kar1-1Vallen et al. (1992)
SEY6210MATα ura3-52 his3Δ200 lys2-801 leu2-3,112 trp1Δ901 suc2Δ9Robinson et al. (1988)
  • a Unless otherwise stated, all strains were created as part of this study.

  • b [GRM1-96] indicates the GFP reporter matrix plasmids listed in Table 2.

  • c MATa isolates that are otherwise SEY6210 isogenic.