Yeast strains used in this study

JIY49h+ ade6-216 cbh2Δ::hisG-ura4+-hisG leu1-32 ura4-D18This study
JIY50h- ade6-216 cbh2Δ::hisG-ura4+-hisG leu1-32 ura4-D18This study
Δmad-2h+ ade6-210 leu1-32 mad2Δ::ura4+ ura4D18S. Sazer
mis-6h- mis6-302 leu1-M. Yanagida
PJ69-4AMATa GAL2-ADE2 gal4Δ gal80Δ his3-200 leu2-3,112 LYS2::GAL1-HIS3 met2::GAL7-lacZ trp1-901 ura3-52P. James
SBP072098-21B7ch- ade6-704 cbh1Δ::hisG-ura4+-hisG leu1-32 ura4-294Lab collection
SBP072198-1Cch- abp1Δ::ura4+ ade6-216 cbh1Δ::hisG-ura4+-hisG leu1-32 ura4-D18Lab collection
SBP082996ch- abp1Δ::ura4+ ade6-704 leu1-32 ura4-294Lab collection
SBP32590ch- ade6-704 leu1-32 ura4-294Lab collection
SBPD022198-15h+/h- ade6-216/ade6-210 leu1-32/leu1-32 ura4-D18/ura4-D18Lab collection
Sp223h- ade6-216 leu1-32 ura4-294Lab collection
Spx33-5Bch- ade6-704 abp1Δ::ura4+ cbh2Δ::hisG-ura4+-hisG leu1-32 ura4-This study
Spx34-6Ach? ade6-704 cbh2Δ::hisG-ura4+-hisG leu1-32 ura4-This study
Spx34-7Ach? ade6-704 cbh1Δ::hisG-ura4+-hisG cbh2Δ::hisG-ura4+-hisG leu1-32 ura4-This study
Spx34-10ch? ade6-704 leu1-32 ura4-This study
Spx34-13ch? ade6-704 cbh1Δ::hisG-ura4+-hisG leu1-32 ura4-This study
Spx40-E9ch- ade6-704 mis6-302This study
Spx47-1ch? ade6-704 cbh2Δ:hisG-ura4+-hisG mis6-302This study
  • a All strains are S. pombe except PJ69-4A, which is S. cerevisiae. Plasmid-bearing strains are described in materials and methods.

  • b Strains designated ura4- carry either the 294 or D18 allele. Strains designated h? were not tested for mating.

  • c These strains contain the 78-kb linear minichromosome pSp(cen1)-7L-sup3E (cen1+ sup3E+ ura4+ TRP1 URA3; Hahnenberger et al. 1991).