TABLE 3

Allelic and ectopic meiotic recombination are uniformily reduced in mum2 mutants

Arg prototrophs (×104)
Location of
Arg allelesa
MUM2/MUM2mum2/mum2
MitoticbMeioticcMitoticMeiotic
HISHIS40.32 ± 0.06 (1.0)220 ± 70 (1.0)0.17 ± 0.09 (0.5)4.5 ± 1.2 (0.02)
PUT2PUT20.26 ± 0.12 (1.0)140 ± 30 (1.0)0.10 ± 0.02 (0.4)2.6 ± 0.6 (0.02)
URA3URA30.13 ± 0.03 (1.0)97 ± 46 (1.0)0.07 ± 0.02 (0.5)0.71 ± 0.34 (0.01)
HIS4LEU20.28 ± 0.05 (1.0)240 ± 20 (1.0)0.19 ± 0.07 (0.7)4.4 ± 1.0 (0.02)
HIS4PUT20.14 ± 0.02 (1.0)26 ± 1 (1.0)0.08 ± 0.03 (0.6)1.3 ± 0.4 (0.05)
URA3HIS40.11 ± 0.01 (1.0)76 ± 13 (1.0)0.05 ± 0.01 (0.5)1.1 ± 0.6 (0.01)
Embedded Image d0.25 ± 0.04 (1.0)84 ± 23 (1.0)0.13 ± 0.05 (0.5)1.8 ± 0.8 (0.02)
LEU2PUT20.16 ± 0.06 (1.0)76 ± 11 (1.0)0.05 ± 0.03 (0.3)1.2 ± 0.2 (0.02)
URA3LEU20.14 ± 0.05 (1.0)55 ± 11 (1.0)0.07 ± 0.03 (0.5)0.9 ± 0.4 (0.02)
URA3PUT20.20 ± 0.17 (1.0)12 ± 2 (1.0)0.03 ± 0.01 (0.2)0.18 ± 0.07 (0.02)
  • a The first locus designated carries the arg4-nsp allele and the second locus carries the arg4-bgl allele in a hemizygous state.

  • b Mitotic recombination frequency ± SD represents the median frequency of a minimum of three independent cultures. Number in parentheses is the frequency normalized to the frequency of the corresponding wild-type strain.

  • c The meiotic cultures were plated after 15 hr in sporulation medium and represent the mean value ± the SD from a minimum of three independent cultures. The number in parentheses is the frequency normalized to the freqeuncy of the corresponding wild-type strain.

  • d Embedded Image represents→homozygosis of the arg4 alleles insertion as described in materials and methods.