TABLE 2

mum2 recombination is dependent on the meiotic recombination machinery and SC components

Ura prototrophs (×107)
StrainRelevant genotypeMitoticaMeioticb
Y1168Wild type6.7 ± 2.4 (1.0)712.0 ± 126 (1.00)
Y1171mum24.7 ± 2.7 (0.7)382.0 ± 69 (0.54)
Y1105spo119.0 ± 1.4 (1.3)7.6 ± 2.2 (0.01)
Y1106spo11 mum27.5 ± 0.7 (1.1)8.0 ± 1.4 (0.01)
Y2900rec1047.3 ± 1.1 (1.1)7.7 ± 2.3 (0.01)
Y2901rec104 mum25.6 ± 2.1 (0.8)8.3 ± 1.5 (0.01)
Y1162hop17.1 ± 2.3 (1.0)12.1 ± 1.7 (0.02)
Y1163hop1 mum26.2 ± 3.3 (0.9)4.1 ± 3.1 (0.01)
Y1169red18.8 ± 3.1 (1.3)111.0 ± 38 (0.15)
Y1170red1 mum24.9 ± 2.9 (0.7)9.2 ± 2.9 (0.01)
  • a Mitotic recombination frequency ± SD represents the median frequency of a mimimum of three independent cultures. Number in parentheses is the frequency normalized to the frequency of the wild-type strain, Y1168.

  • b The meiotic cultures were plated after 15 hr in sporulation medium and represent the mean value ± the SD from a minimum of three independent cultures. The number in parentheses is the frequency normalized to the frequency of the wild-type strain.