TABLE 1

Yeast strains

StrainGenotype
Y1244MATa leu2-27 his4-260 trp1-1 ura3-1 ADE2 arg4::Kan thr1-4 lys2
Y1245MATα leu2-3,112 his4-280 trp1-1 ura3-1 ade2 arg4::Kan THR1 lys2
Y1541Embedded Image
Y1542Y1541 mum2Δ::LYS2/mum2Δ::LYS2
Y1281aY1541 his4::URA3∙arg4-nsp/his4::URA3∙arg4-bgl
Y1275Y1541 mum2Δ::LYS2/mum2Δ::LYS2 his4::URA3∙arg4-nsp/his4::URA3∙arg4-bgl
Y1285Y1541 PUT2::URA3∙arg4-nsp/PUT2::URA3∙arg4-bgl
Y1279Y1541 mum2Δ::LYS2/mum2Δ::LYS2 PUT2::URA3∙arg4-nsp/PUT2::URA3∙arg4-bgl
Y1314Y1541 ura3::URA3∙arg4-nsp/ura3::URA3∙arg4-bgl
Y1318Y1541 mum2Δ::LYS2/mum2Δ::LYS2 ura3::URA3∙arg4-nsp/ura3::URA3∙arg4-bgl
Y1282Y1541 leu2-27 his4::URA3∙arg4-nsp/lue2::URA∙arg4-bgl his4-280
Y1276Y1541 mum2Δ::LYS2/mum2Δ::LYS2 leu2-27 his4::UUA3∙arg4-nsp/leu2::URA∙arg4-bgl his4-280
Y1283Y1541 his4::URA3∙arg4-nsp/his4-280 PUT2/PUT2::URA3∙arg4-bgl
Y1277Y1541 mum2Δ::LYS2/mum2Δ::LYS2 his4::URA3∙arg4-nsp/his4-280 PUT2/PUT2::URA3∙arg4-bgl
Y1315Y1541 his4-260/his4::URA3∙arg4-bgl ura3::URA3∙arg4-nsp/ura3-1
Y1319Y1541 mum2Δ::LYS2/mum2Δ::LYS2 his4-260/his4::URA3∙arg4-bgl ura3::URA3∙arg4-nsp/ura3-1
Y1284Y1541 leu2::URA3∙arg4-nsp/leu2-3,112 PUT2/PUT2::URA3∙arg4-bgl
Y1278Y1541 mum2Δ::LYS2/mum2Δ::LYS2 leu2::URA3∙arg4-nsp/leu2-3,112 PUT2/PUT2::URA3∙arg4-bgl
Y1316Y1541 leu2-27/leu2::URA3∙arg4-bgl ura3::URA3∙arg4pnsp/ura3-1
Y1320Y1541 mum2Δ::LYS2/mum2Δ::LYS2 leu2-27/leu2::URA3∙arg4-bgl ura3::URA3∙arg4-nsp/ura3-1
Y1317Y1541 ura3::URA3∙arg4-nsp/ura3-1 PUT2/PUT2::URA3∙arg4-bgl
Y1321Y1541 mum2Δ::LYS2/mum2Δ::LYS2 ura3::URA3∙arg4-nsp/ura3-1 PUT2/PUT2::URA3∙arg4-bgl
Y3153Y1541 mum2Δ::LYS2/mum2Δ::LYS2 his4::URA3∙arg4-bgl/his4::URA3∙arg4-bgl ura3::URA3∙arg4-nsp/ura3::URA3∙arg4-nsp
Y3154Y1541 mum2Δ::LYS2/mum2Δ::LYS2 his4::URA3∙arg4-bgl/his4::URA3∙arg4-bgl ura3::URA3∙arg4-nsp/ura3::URA3∙arg4-nsp + MUM2 CEN4 TRP1
Y1679Y1541 orc2-1/orc2-1
Y2032Y1541 orc2-1/orc2-1 mum2Δ::LYS2/mum2Δ::LYS2
Y1243Y1541 pol1-17/pol1-17
Y1287Y1541 pol1-17/pol1-17 1 mum2Δ::LYS2/mum2Δ::LYS2
Y1168bEmbedded Image
Y898cY1168 MUM2/mum2Δ::LYS2
Y1171Y1168 mum2Δ::LYS2/mum2Δ::LYS2
Y1105Y1168 spo11::TRP1/spo11::TRP1
Y1106Y1168 mum2Δ::LYS2/mum2Δ::LYS2 spo11::TRP1/spo11::TRP1
Y2900Y1168 rec104::LEU2/rec104::LEU2
Y2901Y1168 mum2Δ::LYS2/mum2Δ::LYS2 rec104::LEU2/rec104::LEU2
Y1162Y1168 hop1::LEU2/hop1::LEU2
Y1163Y1168 mum2Δ::LYS2/mum2Δ::LYS2 hop1::LEU2/hop1::LEU2
Y1169Y1168 red1::LEU2/red1::LEU2
Y1170Y1168 mum2Δ::LYS2/mum2Δ::LYS2 red1::LEU2/red1::LEU2
  • a Nomenclature from Wu and Lichten (1995).

  • b JB128, obtained from Bharagava et al. (1992).

  • c Y898 was described in Engebrecht et al. (1998).