TABLE 2

Oligonucleotide primers used for gene deletions

Name of primerSequence of primera
S1-RHO3Δ1ACATCCATGACATCTTCGTGGACAACCAGCACATCACGCTGAGCCgctagggataacagggtaat
S2-RHO3Δ1CAAGTCGCACTTCAGCGCCACTAGCACCAGCTTCACGCCCTCGCAaggcatgcaagcttagatct
SI-RHO3 Δ 2 CTGGCATCAGAGGAAGCTCCCACCACCAAGCTCTACAAACACAAgctagggataacagggtaat
S2-RHO3Δ2ATTATATTAGTATAGTCTAAAGTTGCAGGCAGTGGGTATTAAAGTaggcatgcaagcttagatct
S1-RHO1GTCGGAGATGGTGCATGCGGGAAAACATGTCTTTTGATTGTGTTTgctagggataacagggtaat
S2-RHO1GCAGATCAACACAACATTGGAGTCTGGGTACGATAACGGCCGTAGaggcatgcaagcttagatct
S1-CDC42Δ1GAGGACTACGACAGGTTGCGGCCGTTGTCGTACCCGTCGACGGACGTGTTTCTCGTGTGCTTCAGCGgctagggataacagggtaat
S2-CDC42Δ1GCAGGGTACGCCCGGGCAGTGGTGATGTACCTCGGGGAACCACTTCTCCTTGACGTTCTCGAACGACaggcatgcaagcttagatct
S1-CDC42Δ2GCAGACATTGAAGTGCGTGGTCGTTGGGGACGGAGCTGTCGGCAAGgctagggataacagggtaatacag
S2-CDC42Δ2CACAAGATGGTGCACTTCTTGCTCTTCTTGATGACCGGCGGTTCGActgcaggcatgcaagcttagatc
S1 -CDC24 ACTTCTTCAAGCTTTATGAGCCGTGGTCTATTGGGCAAAATGCCGCAAgctagggataacagggtaat
S2-CDC24CACAAGATGGTGCACTTCTTGCTCTTCTTGATGACCGGCGGTTCGActgcaggcatgcaagcttagatc
  • a Uppercase sequences correspond to those immediately upstream or downstream of the target locus. Lowercase sequences correspond to terminal regions of the GEN3 selectable marker used in the PCR to generate the disruption cassettes. All sequences are written from 5′ to 3′.