TABLE 3

Cloning of Efr mutants

Complementation
group
No. of
alleles
Gene
disrupted
Position of
transposon
relative to
start codona
EFR14BEM23033/6503
EFR22SPT71750/3998
EFR31YMR212cSee noteb
EFR51GCR13013/3108
EFR61SRB5-15/923
EFR71HFI1358/1466
EFR81BCK11789/4436
  • a When more than one allele was identified, the transposon position is reported for the allele used in subsequent experiments.

  • b We had identified UV-induced alleles of EFR3 in an earlier screen and generated an efr3Δ strain during the course of those experiments. The transposon-insertion allele of EFR3 was identified by noncomplementation of this strain and was not characterized further. The deletion allele is used in subsequent experiments.