TABLE 2

Plasmids used in this study

PlasmidContentsSource
EB0009pRS316, URA3 ARS/CENSikorski and Hieter (1989)
EB0049GPD1::PHO80 URA3 2μLab strain collection
EB0053pAC800, pUC18 PHO80Toh-e and Shimauchi (1986)
EB0098pJJ215, pUC18-HIS3Jones and Prakash (1990)
EB0103pJJ248, pUC18-TRP1Jones and Prakash (1990)
EB0128GPD1::PCL1-HA3 URA3 2μThis study
EB0149pcl1Δ::HIS3Espinoza et al. (1994)
EB0226pBA634, pcl2Δ::URA3Measday et al. (1994)
EB0327pRS316 PHO85.Py2 URA3 ARS/CENThis study
EB0373pFHE27, pKS PCL1Hernan Espinoza
EB0459pJO21, YEp24 CLN2 URA3 2μOgas et al. (1991)
EB0727pKS pcl9Δ::TRP1This study
MP0030pUC18 efr3Δ::LEU2This study
MP0054pKS pho85Δ::TRP1This study
MP0054pKS pho85Δ::TRP1This study
MP0056pRS313 PHO85Δ.Py2 HIS3 ARS/CENThis study (from EB0327)
MP0073pKS pho85-6.Py2 URA3This study (from EB0327)
MP0075pKS pho85-9.Py2 URA3This study (from EB0327)
MP0076pKS pho85-10.Py2 URA3This study (from EB0327)
MP0107pBA945a, pRSETB-PCL10Measday et al. (1997)
MP0108pBA946a, pRSETC-PCL8Measday et al. (1997)
MP0111pBA950, pRSETB-PCL9Measday et al. (1997)
MP0112pBA619, PCL2 LEU2 2μBrenda Andrews
MP0113PBA904, pRS426 CLG1 URA3 2μBrenda Andrews
MP0114pBA906, PCL5 TRP1 2μBrenda Andrews
MP0115pRS426 PHO80 URA3 2μThis study (from EB0053)
MP0117pRS425 PCL6 LEU2 2μAnita Sil
MP0120pRS426 PCL1 URA3 2μThis study (from EB0373)
MP0121pRS426 PCL2 URA3 2μThis study (from MP0112)
MP0126pRS426 PCL5 URA3 2μThis study (from MP0114)
MP0127pRS426 PCL6 URA3 2μThis study (from MP0117)
MP0133YEp13 PCL7 LEU2 2μNasmyth and Tatchell (1980)
MP0134YEp13 PCL9 LEU2 2μNasmyth and Tatchell (1980)
MP0136pKS+ PHO85-5::GAL1-10::PHO85 URA3This study
MP0143CY676, YCp50 PKC1 R398P URA3 ARS/CENNonaka et al. (1995)
MP0145pRS426 PCL7 URA3 2μThis study (from MP0133)
MP0146pRS426 PCL9 URA3 2μThis study (from MP0134)
MP0154YEp13 PCL8 LEU2 2μNasmyth and Tatchell (1980)
MP0155YEp13 PCL10 LEU2 2μNasmyth and Tatchell (1980)
MP0158pRS426 PCL8 URA3 2μThis study (from MP0154)
MP0159pRS426 PCL10 URA3 2μThis study (from MP0155)
pFV-17GAL1-10::FLP1Volkert and Broach (1986)