TABLE 6

Viable progeny from crosses of a haploid 4GI strain

Phenotypes of progenyNo.Proposed genotypeAc17
phenotype
Act2
phenotype
Nit1+ Nit2+; partially flagellateda274GI::NIT1 NIT2 rgn1-13 Ac17-12 Act2-
24 Ac17+15 Act2+
Nit-; fully flagellatedb83nit1 nit2 rgn1-1 or nit1 nit2 RGN118 Ac17-43 Act2-
65 Ac17+40 Act2+
Nit1- Nit2+; fully flagellatedb5nit1 NIT2 rgn1-1 or nit1 NIT2 RGN15 Ac17+2 Act2-
3 Act2+
Nit-; partially flagellateda14GI::NIT1 nit2 rgn1-11 Ac17-1 Act2-
  • a These progeny were used to generate the data in Table 2 for bld2-4 rgn1-1. Less than 9% of the progeny have any flagella.

  • b Greater than 95% of the cells have two flagella.