TABLE 6

Classification of 5-FOAr Leu+ Ade+ clones (A) and 5-FOAr Leu+ Ade- clones (B) based on the copy number and the size abnormality of chromosome III

Copy number
Authentic
chromosome III
Aberrant
chromosome III
No. of clones
identified
No. of cultures
identified
Ratio
(%)
Frequency
(×10-5)
A. 5-FOAr Leu+ Ade+ clones
20Class B116110.039
11Class C878890.31
Total9881000.35
B. 5-FOAr Leu+ Ade- clones
20Class B1157824.0
11Class C185130.63
10Class A735.00.25
Total14071004.9
  • For 5-FOAr Leu+ Ade+ clones (A), 98 clones were randomly chosen from eight independent cultures and analyzed with PFGE and Southern blotting as described in materials and methods. For 5-FOAr Leu+ Ade- clones (B), 140 isolates from seven independent cultures were analyzed in a similar way. The frequency of each class was estimated by multiplying the frequency of total 5-FOAr Leu+ Ade+ cells (0.35 × 10-5) or of total 5-FOAr Leu+ Ade- cells (4.9 × 10-5) by the corresponding ratio, respectively.