Frequency (×10^{5}) | Ratio (%) | ||
---|---|---|---|
Phenotypes | Mean ± SD | Median | |
5-FOA^{r} | 17 ± 14 | 12 | 100 |
5-FOA^{r} Leu^{+} | 7.2 ± 6.3 | 5.2 | 43 |
5-FOA^{r} Leu^{+} Ade^{+} | 0.56 ± 0.65 | 0.35 | 2.9 |
5-FOA^{r} Leu^{-} | 9.8 | 6.8 | 57 |
5-FOA^{r} Leu^{+} Ade^{-} | 6.6 | 4.9 | 41 |
The frequencies of the cells converted to 5-FOA^{r}, 5-FOA^{r} Leu^{+}, and 5-FOA^{r} Leu^{+} Ade^{+} were measured with 15 independent cultures. Cells were inoculated at ̃10^{3} cells into 5 ml medium and the cultures were incubated for an average of 14.2 ± 0.3 generation times. The frequency of 5-FOA^{r} Leu^{-} clones was calculated by subtracting the frequency of 5-FOA^{r} Leu^{+} clones from that of 5-FOA^{r} cells. Similarly, the frequency of 5-FOA^{r} Leu^{+} Ade^{-} clones was calculated by subtracting the frequency of 5-FOA^{r} Leu^{+} Ade^{+} clones from that of 5-FOA^{r} Leu^{+} (see materials and methods).