TABLE 5

Frequency of 5-FOAr convertants from the parent strain RD301 and the association of these convertants with Leu and Ade markers

Frequency (×105)Ratio
(%)
PhenotypesMean ± SDMedian
5-FOAr17 ± 1412100
5-FOAr Leu+7.2 ± 6.35.243
5-FOAr Leu+ Ade+0.56 ± 0.650.352.9
5-FOAr Leu-9.86.857
5-FOAr Leu+ Ade-6.64.941
  • The frequencies of the cells converted to 5-FOAr, 5-FOAr Leu+, and 5-FOAr Leu+ Ade+ were measured with 15 independent cultures. Cells were inoculated at ̃103 cells into 5 ml medium and the cultures were incubated for an average of 14.2 ± 0.3 generation times. The frequency of 5-FOAr Leu- clones was calculated by subtracting the frequency of 5-FOAr Leu+ clones from that of 5-FOAr cells. Similarly, the frequency of 5-FOAr Leu+ Ade- clones was calculated by subtracting the frequency of 5-FOAr Leu+ Ade+ clones from that of 5-FOAr Leu+ (see materials and methods).