TABLE 4

Aberrant chromosomes of class C clones from RD101

Size
changea
(kb)
No. of
clones
identified
No. of
cultures
identified
III-205 locus
on the aberrant
chromosomeb
-11011-
-10521-
-10011-
-80159-(ND for 2 clones)
-6072-
-5532-
-5054-(ND for 1 clone)
-4022+
-304c4+
-2511-
-101d1+
+11011-
+14511-
+21011-
+25011-
+34022ND
+38022-
+62011ND
Total519
  • a The length of the aberrant chromosome was determined by PFGE and the size difference between it and the 330-kb wild-type chromosome III is indicated.

  • b Presence (+) or absence (-) of the III-205 locus on the aberrant chromosome was determined by quantifying the locus with PCR as shown in Figure 2. ND, not determined because of loss of the clone during storage.

  • c In two cases out of four, the other normal-sized chromosome III could not be identified (the third subtype in the text).

  • d The normal-sized chromsome III was not identified (the third subtype in the text).