TABLE 3

Frequency of 5-FOAr cells and clones analyzed for their chromosome structure

Mean ± SD (median) × 105 [class distribution (%)]
StrainGenotypeaTotal 5-FOArbClass Ac
chromosome loss
Class Bc
no chromosome change
Class Cc
aberrant chromosome
RD101Embedded Image 20 ± 13 (16)13 ± 9.8 (8.5) [62]6.7 ± 3.7 (6.8) [30]1.1 ± 1.1 (0.71) [8.0]
RD104Embedded Image 16 ± 3.1 (16)5.4 ± 3.4 (4.5) [37]8.8 ± 1.6 (8.5) [60]0.47 ± 0.65 (0.24) [3.2]
RD201Embedded Image 3.4 ± 1.4 (2.9)0.10 ± 0.090 (0.051) [2.6]3.7 ± 1.6 (4.1) [97]<0.022 [<0.56]
RD103Embedded Image <0.055NDNDND
YMH1III-205::URA30.043 ± 0.013NDNDND
  • a Only the locus where the URA3 marker is located in each strain is shown.

  • b The 5-FOAr frequency was determined with 23, 13, 10, 9, and 6 independent cultures for RD101, RD104, RD201, RD103, and YMH1, respectively.

  • c The frequencies of classes A-C are averages of those calculated for each culture from the ratios of the corresponding clones structurally analyzed as in the text. Total number of clones and cultures analyzed for the measurement was 224 clones from 6 cultures for RD101, 134 clones from 7 cultures for RD104, and 152 clones from 5 cultures for RD201. For RD101, an additional 416 clones from 12 independent cultures were analyzed for their chromosome structure and were not used to calculate the frequencies. Class distribution in each strain was listed in brackets. ND, not determined for RD103 and YMH1.