TABLE 2

Nucleotide sequences of primers

NamePriming positions (origin)aStrandNucleotide sequencesb
dp416W-B152-175 (pRS416)WattaagcgcgcGGCATCAGAGCAGATTGTACTGAG
dp416C-B1309-1338 (pRS416)WggtatgcgcgcCTTACGCATCTGTGCGGTATTTC
dAFh505-27 to -8 (pNKY51)WatggattctagaacagttggtatattgggagggggacaattgggacgtatgattgttgagCACGAGGCCCTTTCGTCTTC
dARh22143845-3861 (pNKY51)WcttgttttctagataacgttcgtaaccgacagtttctaacttttgtgctttgacaagaacCGATGCGTCCGGCGTAG
d3W205-X204 (chr. III)WtggcctctagATTTTAGTGCGATTTTAGAATAACTCTTGG
d3C205-B205 (chr. III)WattacgcgcgCAATGAAATAATATGTATATGTCTACATAGAG
d3W205-B205 (chr. III)WttattgcgcgCTTTTTCTAATAAACGAGGAAAATGG
d3C205-M205 (chr. III)WggatgcccggGCTCCACTTGATGCTTCCTTATTG
d3W102102 (chr. III)WTGGTTCGGTTGGTGCTTAGG
d3C102102 (chr. III)WTCGTTATAATCAAAGGCGTGAGG
d3W205205 (chr. III)WAACCCCACTTACCCCTCTGTC
d3C205-R205 (chr. III)WTAAATTGGTGTCCCAGCAGC
d3C205205 (chr. III)WGGCAACAGTAGGCAGTGAAAGC
d3W197197 (chr. III)WAACTATGTCTGCAAACAGTTCTTGG
d3C294294 (chr. III)WCATTATCAATCCTTGCATCCAGC
d3W5454 (chr. III)WCTCACACGATCTGACTGTGACC
d3C5454 (chr. III)WCGTAAATGGTAACATCTTCGCTG
d3W313-E313 (chr. III)WattggtgagaattcACAGTCCAGAAGGTTACGTCATC
d3C313-BK314 (chr. III)WcgtacgggagatctcgcggtaccTTCCGCTAGGAAGAATGTGC
d3W315-BS314 (chr. III)WcgcgcacaagatctgcggagctcGACATATTAACGTAGGCCCGTACG
d3C315-S315 (chr. III)WctccaattgcatgcATCCAACTCCACCACCACTTAC
d3W9191 (chr. III)WTCTAAGGCGCCTGATTCAAG
d3C9292 (chr. III)WCCAGTTCTGATACCTGCATCC
d3W312312 (chr. III)WCTCATACGATGGAGCTCGTTC
d3C314314 (chr. III)WCGTACGGGCTACGTTAATATGTC
d5W116116 (chr. V)WTAAGGAACGTGCTGCTACTACTCATCC
d5C117117 (chr. V)WGTTACTTGGTTCTGGCGAGG
d5W230230 (chr. V)WTTCACTTGCCACAACTCAGC
d5C231231 (chr. V)WGGATAATGGCCTCATGGTACC
d5W543543 (chr. V)WCTATTGCCAACTTCTGCGC
d5C544544 (chr. V)WGCCTCTGATGATTGTGTAGCC
d10W587587 (chr. X)WAACGCTTCTAACACATCACGC
d10C588588 (chr. X)WAGCCTTCTATGCTGGAGTCG
d15W564-S564 (chr. XV)WatttgggagctcCAAAGATATCCTGACGTAGCGC
d15C566-A566 (chr. XV)WatttgggcccTCATGATTGATTATTACTGCTATGCTG
  • W, Watson strand; C, Crick strand.

  • a Numbers represent the endpoints of the priming sequences of the corresponding primer. For dp416W-B and dp416C-B, numbering is as in the GenBank database. For dAFh505 and dArh2214, numbering is as by Alani et al. (1987). Numbers of other primers represent the approximate positions in a kilobase scale according to the SGD database. Chromosomes and plasmids where the primer sequence is located are indicated in parentheses.

  • b Nonannealing tail sequences are in lowercase and priming sequences are in uppercase. Sequences corresponding to restriction sites added are underlined.