TABLE 2

Yeast two-hybrid interactions for trichome regulators based on β-galactosidase lift assay results

DNA binding domain constructs
Activation domain fusion constructspGL1BpGL1NTBpTTG1BpTTG1MBpGL3CTB
pGL3A+++++++
pGL3NTA++++++
pGL3CTA+
pGL396A+++
pGL3211A+++
pGL1NTA
pTTG1A

Constructs and yeast strains are as in materials and methods and Figure 3. ++, strong β-galactosidase activity was detected in 1 hr or less. +, activity was faint within the first 3 hr and dark blue within 10 hr. –, no activity could be detected in >10 hr.