Leaf trichome phenotypes for wild-type, mutant, and transgenic Arabidopsis lines
Branching phenotype | ||||||
---|---|---|---|---|---|---|
Genotype | Leaf number | 1 | 2 | 3 | 4 | Total no. of trichomes |
Ler wild type | 1,2 | 0.1 ± 0.31 | 7.8 ± 2.5 | 3.9 ± 2.0 | 11.8 ± 2.0 | |
n = 15 | 3 | 0.27 ± 0.59 | 25.5 ± 5.7 | 17.8 ± 5.4 | 43.7 ± 5.9 | |
4 | 0.2 ± 0.56 | 31.9 ± 5.6 | 23.7 ± 6.6 | 55.8 ± 6.3 | ||
gl3-l | 1,2 | 0.59 ± 1.0 | 4.2 ± 1.9 | 0.32 ± 0.64 | 5.1 ± 2.1 | |
n = 11 | 3 | 4.5 ± 1.8 | 14.6 ± 2.9 | 0.45 ± 0.52 | 19.5 ± 3.3 | |
4 | 9.3 ± 4.1 | 19 ± 5.0 | 0.18 ± 0.60 | 28.5 ± 8.3 | ||
gl3-2 | 1,2 | 0.45 ± 0.6 | 10.3 ± 4.0 | 1.9 ± 1.7 | 12.2 ± 4.9 | |
n = 10 | 3 | 5.4 ± 3.1 | 28.3 ± 8.1 | 3.5 ± 1.4 | 37.2 ± 10 | |
4 | 7.6 ± 4.8 | 35.3 ± 10 | 2.0 ± 1.7 | 44.9 ± 14 | ||
gl3-1XLer wt | 1,2 | 0.07 ± 0.23 | 0.71 ± 0.91 | 12.6 ± 3.2 | 0.36 ± 0.50 | 13.7 ± 2.9 |
n = 7 | 3 | 2.0 ± 1.5 | 39 ± 7.5 | 4.0 ± 3.5 | 45 ± 9.3 | |
4 | 1.6 ± 1.1 | 56.7 ± 11 | 5.1 ± 4.2 | 63.4 ± 11 | ||
1/14/99—3A | 1,2 | 0.63 ± 0.92 | 9.9 ± 2.5 | 0.63 ± 0.74 | 11.1 ± 2.0 | |
n = 4 | 3 | 26 ± 3.7 | 4 ± 0.82 | 30 ± 3.9 | ||
4 | 1.0 ± 1.2 | 41.8 ± 10 | 5.3 ± 4.7 | 48 ± 14 | ||
1/14/99—6A | 1,2 | 0.20 ± 0.41 | 9.6 ± 2.3 | 2.6 ± 2.0 | 12.4 ± 2.1 | |
n = 10 | 3 | 0.20 ± 0.63 | 19.3 ± 4.7 | 11.5 ± 6.8 | 31 ± 4.2 | |
4 | 28.6 ± 5.9 | 12.6 ± 9.9 | 41.2 ± 8.1 | |||
1/14/99—6B | 1,2 | 0.13 ± 0.34 | 0.50 ± 0.93 | 10.4 ± 1.5 | 0.38 ± 0.52 | 11.3 ± 1.6 |
n = 4 | 3 | 25± 2.4 | 2.5 ± 2.6 | 27.5 ± 4.9 | ||
4 | 35.8 ± 2.9 | 3 ± 1.6 | 38.8 ± 3.9 | |||
1/14/99—4A | 1,2 | 0.38 ± 0.52 | 8.0 ± 2.1 | 4.1 ± 3.2 | 12.5 ± 3.3 | |
n = 4 | 3 | 17.8 ± 6.7 | 13.3 ± 9.7 | 31 ± 5.6 | ||
4 | 1.0 ± 2.0 | 31.8 ± 16 | 11.8 ± 12 | 44.5 ± 7.2 | ||
12/21/98—8A | 1,2 | 0.30 ± 0.67 | 8.9 ± 2.1 | 6.3 ± 1.3 | 15.6 ± 2.7 | |
n = 5 | 3 | 0.40 ± 0.89 | 14.8 ± 2.7 | 21 ± 6.2 | 36 ± 7.9 | |
4 | 20.6 ± 5.3 | 23.3 ± 5.0 | 43.8 ± 8.9 | |||
WS wt | 1,2 | 8.7 ± 3.9 | 33.6 ± 5.7 | 42.3 ± 6.3 | ||
n = 5 | 3 | 16.4 ± 4.4 | 92.8 ± 11 | 109 ± 11 | ||
4 | 20.8 ± 4.3 | 106 ± 6.8 | 127 ± 9.8 | |||
4/15/98—17A | 1,2 | 29.8 ± 6.0 | 11.7 ± 6.3 | 41.5 ± 12 | ||
n = 5 | 3 | 59.8 ± 10 | 36.8 ± 12 | 96.6 ± 11 | ||
4 | 80.2 ± 14 | 38 ± 14 | 118 ± 19 | |||
4/15/98—17B | 1,2 | 29.8 ± 3.9 | 11.7 ± 2.7 | 41.5 ± 5.1 | ||
n = 5 | 3 | 62.8 ± 9.6 | 39 ± 8.1 | 101 ± 13 | ||
4 | 75 ± 12 | 38.4 ± 9.9 | 113 ± 22 | |||
4/15/98—17F | 1,2 | 28.1 ± 9.1 | 15.9 ± 6.3 | 44 ± 8.1 | ||
n = 5 | 3 | 43.6 ± 19 | 26.3 ± 12 | 68.7 ± 30 | ||
4 | 65.2 ± 13 | 36.8 ± 8.5 | 102 ± 17 |
Because trichome phenotypes on the first two leaves are indistinguishable, data were pooled. Consequently, n for leaf 1,2 is twice the n given for the number of plants. Branch phenotype refers to the numbers of processes on the trichome. The number 1 refers to an unbranched spike. The number 2 is a two-branched trichome, etc. 1/14/99—3A, 6A, 6B, 4A, and 12/21/98—8A are gl3-1 transformed with the GL3 genomic clone, pD2LX. 4/15/98—17A, 17B, and 17F are WS wild type transformed with the GL3 antisense clone, pEZF1. n, number of individual plants counted for each strain.