TABLE 2

Eicosenoic acid (20:1) levels in 5-day-old seedlings

ClassPlant% 20:1 vs. total fatty acidsAmount of 20:1 (pg/seedling)Fresh weight (mg/seedling)
Wild typeCol-01.8 ± 0.450 ± 160.99 ± 0.09
Wild typeWs5.7 ± 3.1150 ± 910.71 ± 0.09
1B712.5 ± 3.5680 ± 4300.59 ± 0.02
1B118.1 ± 0.8270 ± 400.58 ± 0.03
1B1714.0 ± 3.5690 ± 5400.48 ± 0.02
1B5215.1 ± 3.3650 ± 3400.53 ± 0.04
1B298.6 ± 2.8230 ± 1000.49 ± 0.05
1B4011.8 ± 2.5530 ± 2200.42 ± 0.07
1B44 (pex5-1)8.8 ± 1.3270 ± 530.55 ± 0.06
2 ibr1-1 3.7 ± 1.599 ± 430.71 ± 0.06
2 ibr1-2 6.7 ± 2.7210 ± 1300.60 ± 0.05
2 ibr2 4.8 ± 1.3130 ± 400.72 ± 0.04
2 ibr3 4.2 ± 0.8110 ± 400.63 ± 0.06
3 ibr4 6.5 ± 2.5140 ± 600.67 ± 0.08
4 ibr5 1.6 ± 0.0133 ± 20.76 ± 0.05
4 ibr6 3.3 ± 1.894 ± 630.90 ± 0.10

Values represent the mean ± standard deviation of 3 samples of 75 seedlings. All mutants are in the Col-0 background except for B52 and ibr4, which are in the Ws background. The percent eicosenoic acid (20:1) was calculated by dividing the area of the 20:1 GC peak by the total area of fatty acid peaks. The amount of 20:1 was calculated by multiplying the area of the 20:1 peak by the amount of standard added and dividing by the area of the standard peak. Fresh weights were measured immediately before processing.