lin-25 is involved in P12 cell fate specification

GenotypeTemperaturenaP11 → P12b (%)P12 → P11b (%)
N2 (wild type)20°4700
lin-25(ar90) 20°8808
let-23(n1045) 15°80011
let-23(n1045) 20°89012
lin-15(n309) 20°115320
lin-25(ar90);lin-15(n309) 20°5213c6
sur-2(ku9) 20°7206

Alleles used are as follows: lin-25(ar90), which strongly reduces or eliminates gene activity; lin-25(n545ts) and lin-25(n1722ts) appear to have reduced gene activity at 15° and 20°;8% of lin-25(n545ts) mutant hermaphrodites are Egl at 15° (Ferguson and Horvitz 1985) and 2% of lin-25(n1722ts) mutant hermaphrodites are Egl at 20° (Tuck and Greenwald 1995); lin-15(n309) is most likely a null allele (Clark et al. 1994).

  • a Number of animals examined.

  • b The fates of P11 and P12 were assessed using the criteria of Jiang and Sternberg (1998).

  • c Four animals of this genotype had two cells with the morphology of and one of P11.p. lin-25 mutations sometimes cause Pn.p cells that later become part of the vulva equivalence group to divide during the L1 stage to give rise to two cells that both become VPCs. One possibility is that in the four animals with two cells and one P11.p cell seen here, P11 or P12 divided to give rise to two cells that subsequently both became part of the P11/P12 equivalence group.