TABLE 3

Effect of mutations in git3 and gpa2 on fbp1-lacZ expression

StrainRelevant genotypeβ-Galactosidase activity
RepressedDerepressed
RWP9 git3 Δ 1501 ± 2413363 ± 390
CHP459 gpa2 Δ 1403 ± 2142800 ± 100
RWP22git3Δ gpa2Δ2350 ± 2983165 ± 97
RWP32agit3Δ gpa2R176H7 ± 0491 ± 61

β-Galactosidase activity was determined from two to four independent cultures as described in materials and methods. The average ± SE represents specific activity per milligram soluble protein.

  • a Strain RWP32 is 5-FOAR due to suppression of the git3 deletion by gpa2R176H.