TABLE 2

Suppression of git3 and git5 mutations by gpa2R176H

StrainRelevant genotypeβ-Galactosidase activity
RepressedDerepressed
FWP112Wild type20 ± 41666 ± 21
CHP14 git3-14 760 ± 682815 ± 256
CHP478 git5 Δ 1050 ± 782291 ± 179
RWP4 gpa2R176H 5±1442 ± 167
RWP39 git3-14 gpa2R176H 5±0354 ± 145
RWP30git5Δ gpa2R176H8 ± 2510 ± 249

β-Galactosidase activity was determined from two to six independent cultures as described in materials and methods. The average ± SE represents specific activity per milligram soluble protein.