TABLE 1

Yeast strains used in this study

StrainGenotypeReference
JN516MATα his3-11, 15 leu2-3, 112 ura3-52 trp1-Δ1lys2 SSA1 ssa2::LEU2 ssa3::TRP1 ssa4::LYS2Becker et al. (1996)
AJMY01JN516 with pYES2(URA3)This study
AJMY02JN516 with pYES2-(His)6-SSA1This study
AJMY03JN516 with pYES2-(His)6-ssa1-101 (K69Q)This study
AJMY04JN516 with pYES2-(His)6-ssa1-102 (G198D)This study
AJMY05JN516 with pYES2-(His)6-ssa1-103 (G199D)This study
AJMY06JN516 with pYES2-(His)6-ssa1-104 (G226D)This study
JB67MATα his3-11, 15 leu2-3, 112 ura3-52 trp1-Δ1lys2 ssa1::ssa1-45 ssa2::LEU2 ssa3::TRP1ssa4::LYS2Becker et al. (1996)
AJMY07JB67 with pYES2(URA3)This study
AJMY08JB67 with pYES2-(His)6-SSA1This study
AJMY09JB67 with pYES2-(His)6-ssa1-101 (K69Q)This study
AJMY10JB67 with pYES2-(His)6-ssa1-102 (G198D)This study
AJMY11JB67 with pYES2-(His)6-ssa1-103 (G199D)This study
AJMY12JB67 with pYES2-(His)6-ssa1-104 (G226D)This study
MW332MATα his3-11, 15 leu2-3, 112 lys2 ura3-52 trp1-Δ1 ssa1::HIS3 ssa2::LEU2 ssa3::TRP1 ssa4::LYS2 pGAL-SSA1 (URA3)E. Craig
JN515MATa his3-11, 15, leu2-3, 112 ura3-52 trp1-Δ1lys2 SSA1 ssa2-1 ssa3-1 ssa4-2E. Craig
AJMY22MATa/MATα his3-11, 15/his3 leu2-3, 112/leu2 lys2/lys2 ura3-52/ura3 trp1-Δ1trp1SSA1/ssa1::HIS3 ssa2-1/ssa2::LEU2ssa3-1/ssa3::TRP1 pGAL-SSA1 (URA3)This study
AJMY23AJMY22 with pYES2-SSA1 (URA3) instead of pGAL-SSA1 (URA3)This study
AJMY28MATα his3 leu2 lys2 Δtrp1 ura3 ssa1::HIS3ssa2::LEU2 ssa3::TRP1 ssa4::LYS2pYES2-(His)6-SSA1 (URA3)This study
AJMY28AAJMY28, but lys2This study
AJMY28A1AJMY28A with pRS317 (LYS2)This study
AJMY28A2AJMY28A with pRS317-GPD-(His)6-SSA1This study
AJMY28A3AJMY28A with pRS317-GPD-(His)6-ssa1-101 (K69Q)This study
AJMY28A4AJMY28A with pRS317-GPD-(His)6-ssa1-102 (G198D)This study
AJMY28A5AJMY28A with pRS317-GPD-(His)6-ssa1-103 (G199D)This study
AJMY28A6AJMY28A with pRS317-GPD-(His)6-ssa1-104 (G226D)This study
MW141MATα his3-11, 15 leu2-3, 112 lys2 ura3-52 trp11ssa1::HIS3 ssa2::LEU2 ssa4::URA3pGAL-SSA1 (TRP1)Deshaies et al. (1988)
AJMY36JN516 with pRS426 (URA3)This study
AJMY37JN516 with pRS426-GPD-(His)6-SSA1This study
AJMY38JN516 with pRS426-GPD-(His)6-ssa1-101 (K69Q)This study
AJMY39JN516 with pRS317-GPD-(His)6-ssa1-103 (G199D)This study
AJMY48JB67 with pCu426 (URA3)This study
AJMY49JB67 with pCu426-(His)6-SSA1This study
AJMY50JB67 with pCu426-(His)6-ssa1-101 (K69Q)This study
AJMY51JB67 with pCu426-(His)6-ssa1-103 (G199D)This study