TABLE 1

Amplification primers used in this study

IntronDirectionPrimer
7 (section 1)Forward5′-TGT ATG CCC TAT GGA TGG AG-3′
Reverse5′-TCT GAT TAT GAT GTG GAT GC-3′
7 (section 2)Forward5′-TTT CCG CCT CTG CTG TAA TC-3′
Reverse5′-TTG TGA AAA TAA TCC TGG TAA G-3′
44Forward (F1)5′-TTG GGG GAA ATA AGG CAA TTC C-3′
Reverse (R1)5′-AGA AGC AAA AGA AGA ACC CCG C-3′
Forward (F4)5′-CTG TAG TAC CAG AAT CTC C-3′
Reverse (R4)5′-CTT GAA GTT GGG GAC ACT AGA G-3′
Forward (F11)5′-ATA ATG AGA ACT ACA AAC CAA G-3′
Reverse (R5)5′-AAC TCT GAA ATC CTA AAC AAA T-3′
  • Approximate positions of primers are shown in Figure 1. Amplification of 3 kb of intron 44 was done with three overlapping fragments using primer pairs F1 + R1, F4 + R4, and F11 + R5.