TABLE 3

Rescue of· ·sec9-4· ·by Sec9p/Spo20p chimeras·

Gene expressedaGrowth at 37°b
SEC9+++
SPO20
PSSS
SPSS
SSPS+++
SSSP+
SPPS
SPSP
SSPP
SPPP
  • a For chimeric genes, S represents SEC9 sequence and P represents SP020 as described in the text.

  • b Integrating plasmids carrying the designated gene under control of the SEC9 promoter were transformed into strain BY41 (sec9-4). −, no growth; +, weak growth; +++, wild-type growth as measured by size of colonies after 3 days of incubation at 37°.