Genetic complementation of elave5 and elavts1 by domain replacement transgenes
elave5 | elavts1 | |||||||
---|---|---|---|---|---|---|---|---|
Transgene | Line | No. rescued males | No. control females | Rescue (%) | No. rescued males | No. control females | Rescue (%) | No. ts1 males |
ER1 | 29 | 26 | 467 | 6 | 219 | 326 | 67 | 0 |
2A | 17 | 455 | 4 | 163 | 252 | 65 | 0 | |
2B | 0 | 930 | 0 | 1 | 861 | 0 | 0 | |
ER12 | 2A | 0 | 436 | 0 | 0 | 346 | 0 | 0 |
2B | 0 | 481 | 0 | 0 | 291 | 0 | 0 | |
52 | 0 | 227 | 0 | 0 | 299 | 0 | 0 | |
ER3 | 2A | 267 | 297 | 90 | 245 | 370 | 66 | 0 |
EH3 | 2A | 222 | 339 | 65 | 342 | 354 | 97 | 2 |
ES1/2 | 3A | 69 | 490 | 14 | 253 | 359 | 70 | 1 |
3B | 122 | 433 | 28 | 228 | 254 | 90 | 13 | |
42 | 80 | 230 | 35 | 145 | 191 | 76 | 3 | |
ES1 | 3A | 549 | 0 | 0 | 26 | 345 | 8 | 3 |
42 | 338 | 0 | 0 | 0 | 310 | 0 | 2 | |
45 | 517 | 0 | 0 | 0 | 310 | 0 | 0 | |
ES2 | 2 | 0 | 514 | 0 | 53 | 312 | 17 | 0 |
2A | 16 | 337 | 5 | 31 | 284 | 11 | 0 | |
3A | 0 | 483 | 0 | 5 | 261 | 2 | 0 | |
ES12 | 3A | 0 | 440 | 0 | 0 | 305 | 0 | 0 |
25 | 0 | 355 | 0 | 0 | 273 | 0 | 0 | |
32 | 0 | 426 | 0 | 0 | 290 | 0 | 0 |
Transgenes encoding mutant proteins were tested for functional complementation with elav alleles: elave5 and elavts1 w/Y; Tg/balancer were crossed to elavxw sn/balancer (x denotes the elav allele, Tg stands for the transgene). Rescue (%) = (number of rescued males of genotype elavx w sn/Y; Tg/+)/(number of control females of genotype elavxw sn/w; Tg/+) × 100. For details see materials and methods.