TABLE 1

Linkage of suppression of U4-cs1 cold sensitivity to fragments of mutagenized PRP8

Mutagenized PRP8 interval	Subregion^{^a}	No. of strains for which suppression was linked to subregion	Fraction of strains tested (%)^{^b}
1	1–253	6	10.2
	254–355	4	6.8
	356–660	27	45.8
	479–660^{^c}	3	25.0^{^d}
	661–753^{^c}	1	8.3^{^d}
2	479–761	6	13.3
	762–913	25	55.6
	914–1213	8	17.7
3	1022–1213	18	35.3
	1597–1942	24^{^e}	55.8^{^e}
4	1597–1942	41	82.0

Linkage of the suppression phenotype to small fragments of Prp8 was tested for selected strains that originated from mutagenizing the four intervals of PRP8.
↵^a Given in amino acids encoded by the amplified fragment.
↵^b Given in percentages per interval.
↵^c To be comparable with the subregion comprising amino acids 356–660, the subregion coding for amino acids 479–753 was divided, and the strains were assigned on the basis of the sequencing result.
↵^d These numbers were obtained from a subpopulation of only 12 strains tested for linkage to amino acids 479–753.
↵^e Eight strains, for which suppression was not linked to amino acids 1022–1213, were not tested for linkage to amino acids 1597–1942.