TABLE 1

Linkage of suppression of U4-cs1 cold sensitivity to fragments of mutagenized PRP8

Mutagenized PRP8 intervalSubregionaNo. of strains for which suppression was linked to subregionFraction of strains tested (%)b
11–253610.2
254–35546.8
356–6602745.8
479–660c325.0d
661–753c18.3d
2479–761613.3
762–9132555.6
914–1213817.7
31022–12131835.3
1597–194224e55.8e
41597–19424182.0
  • Linkage of the suppression phenotype to small fragments of Prp8 was tested for selected strains that originated from mutagenizing the four intervals of PRP8.

  • a Given in amino acids encoded by the amplified fragment.

  • b Given in percentages per interval.

  • c To be comparable with the subregion comprising amino acids 356–660, the subregion coding for amino acids 479–753 was divided, and the strains were assigned on the basis of the sequencing result.

  • d These numbers were obtained from a subpopulation of only 12 strains tested for linkage to amino acids 479–753.

  • e Eight strains, for which suppression was not linked to amino acids 1022–1213, were not tested for linkage to amino acids 1597–1942.