TABLE 1

Changes in CAG repeat tract length in replication mutants

StrainGenotypeTractNo. of repeat unitsNo. of changes among siblings from six parental coloniesTotal no. of changes/no. of siblingsNo. of expansionsNo. of contractionsMean size of expansions ± SD (repeat units)Mean size of contractions ± SD (repeat units)
CAG101Wild typeaC780,0,0,0,1,12/19211838
CAG119Wild typeaD713,5,5,6,6,732/18903237 ± 17
CAG126rth1Δ/rad27ΔaC7813,2033/61141917 ± 1040 ± 15
CAG128rth1Δ/rad27ΔaD7120,2444/628368.9 ± 6.526 ± 15
CAG213cdc9-1bC781,4,4,5,5,625/18716914 ± 1338 ± 19
CAG214cdc9-1D715,6,7,9,9,1248/1908407.5 ± 4.731 ± 15
CAG229cdc9-1C7811,1425/6391614 ± 1125 ± 13
rth1Δ/rad27Δ
CAG215cdc9-2bC782,3,6,6,7,933/190151816 ± 1123 ± 17
CAG216cdc9-2D713,4,6,7,8,1139/1924354.2 ± 2.431 ± 17
CAG221pri2-1bC782,2,2,3,4,619/19161314 ± 1432 ± 15
CAG222pri2-1bD719,10,10,12,12,1568/1885637.4 ± 5.438 ± 16
CAG217dna2-1C780,0,1,1,2,48/191269.5 ± 3.532 ± 11
CAG218dna2-1D715,7,8,8,9,1350/19205033 ± 19
CAG219rnh1ΔC780,0,0,0,0,11/1880127
CAG220rnh1ΔD714,5,6,8,8,1445/1912437.0 ± 5.739 ± 12
CAG226rnh35ΔC780,0,0,0,1,12/1920211 ± 7
CAG227rnh35ΔD712,3,6,6,7,933/19203339 ± 17
CAG230rnh1Δ/rnh35ΔC680,0,1,2,2,38/18417923 ± 16
  • a The data for five of the six wild-type parental colonies and the rth1Δ/rad27Δ data were reported in previous studies (Schweitzer and Livingston 1998, 1999).

  • b Tract instability in these strains was found to be significantly higher (P < 0.008) than that in wild-type cells by a randomization test (Schweitzer and Livingston 1999).