TABLE 3

Rescue of kurtz1 lethality with induced expression of a krz cDNA

GenotypeHeat shockaStage treatedbAdult survivors (%)Total no. of animals
Embedded Image +1st instar6 (14.6)41
Embedded Image 0 (0)57
Embedded Image +1st instar0 (0)25
Embedded Image 0 (0)40
Embedded Image +2nd instar5 (14.7)34
Embedded Image 0 (0)119
Embedded Image +2nd instar0 (0)25
Embedded Image 0 (0)62
Embedded Image +Early 3rd instar27 (27.8)97
Embedded Image 0 (0)150
Embedded Image +Early 3rd instar11 (14.6)75
Embedded Image 0 (0)100
Embedded Image +Late 3rd instar72 (43.6)165
Embedded Image 0 (0)150
Embedded Image +Late 3rd instar27 (54)50
Embedded Image 0 (0)100
Embedded Image +Late 3rd instar0 (0)100
Embedded Image +Late 3rd instar0 (0)100
  • a Larvae were heat shocked at 37° for 1 hr and then returned immediately to 23° (+) or left at 23° (−).

  • b The larvae were selected at the indicated stages for heat-shock or control treatments. Early third instar represents 0–12 hr after the second molt. Late third instar larvae were collected and treated 24–48 hr after the second molt.