TABLE 5

Synergistic interactions occur between sir3-eso alleles and the nat1 mutant

Strain: Relevant genotype:LPY1132 MATα nat1Δ sir3Δ
SIR3 allelebMating efficiencya
SIR32 × 10−1 ± 0.9 (1)
sir3-A2T2 × 10−1 ± 1 (1)
sir3-R30K5 × 10−2 ± 3 (10−1)
sir3-R92K3 × 10−2 ± 2 (10−1)
sir3-L96F3 × 10−2 ± 2 (10−1)
sir3-8(E131K)2 × 10−5 ± 0 (10−4)
sir3-T135I8 × 10−3 ± 6 (10−2)
sir3-E140K3 × 10−2 ± 2 (10−1)
sir3-L208S6 × 10−3 ± 5 (10−2)
sir3-S813F2 × 10−5 ± 0.8 (10−4)
Vector only4 × 10−5 ± 0.1 (10−4)
  • a Mating efficiency is expressed as a mean of two experimental values, with the range indicated. In parentheses, each efficiency is presented relative to this wild-type plasmid control, rounded to the nearest exponent.

  • b Plasmids used were pLP187, pLP189, pLP1131, pLP1048, pLP464, pLP675, pLP946, pLP469, pLP473, pLP472, and pRS313, in descending order.