TABLE 4

A subset of sir3-eso mutants exhibits MATa-specific mating defects

Strain: Relevant genotype:Mating efficiencya
LPY3620 MATasir3ΔLPY3320 MATasir1Δ sir3Δ
SIR3 alleleb
SIR32 × 10−1 ± 0.5 (1)3 × 10−2 ± 2 (10−1)
sir3-A2T3 × 10−1 ± 1 (1)6 × 10−6 ± 3 (10−5)
sir3-R30K2 × 10−1 ± 0.8 (1)≤3 × 10−6 ± 0.7 (10−5)
sir3-R92K7 × 10−2 ± 0.5 (10−1)≤5 × 10−6 ± 1 (10−5)
sir3-L96F2 × 10−1 ± 0.5 (1)≤3 × 10−6 ± 0.5 (10−5)
sir3-8(E131K)2 × 10−1 ± 0.2 (1)2 × 10−5 ± 1 (10−4)
sir3-T135I2 × 10−3 ± 0.4 (10−2)≤3 × 10−6 ± 0.6 (10−5)
sir3-E140K4 × 10−3 ± 2 (10−2)4 × 10−6 ± 0.1 (10−5)
sir3-L208S6 × 10−6 ± 2 (10−5)≤3 × 10−6 ± 0.3 (10−5)
sir3-S813F3 × 10−2 ± 0.5 (10−1)≤3 × 10−6 ± 0.1 (10−5)
ORC1N-SIR3C8 × 10−2 ± 3 (10−1)2 × 10−2 ± 0.3 (10−1)
Vector only3 × 10−5 ± 0.4 (10−4)≤3 × 10−6 ± 0.2 (10−5)
  • a Mating efficiency is expressed as a mean of two experimental values, with the range indicated. In parentheses, each efficiency is presented relative to this wild-type plasmid control, rounded to the nearest exponent.

  • b Plasmids used were pLP187, pLP189, pLP1131, pLP1048, pLP464, pLP675, pLP946, pLP469, pLP473, pLP472, pSIR3.12, and pRS313, in descending order.