TABLE 3

The sir3-eso phenotype is characterized by a mating defect in the sir1 mutant background

Strain: Relevant genotype:Mating efficiencya
LPY3238 MATα sir3ΔLPY3321 MATα sir1Δ sir3ΔLPY3237 MATα sir1Δ
SIR3 alleleb
SIR37 × 10−1 ± 0.7 (1)2 × 10−1 ± 0.3 (10−1)2 × 10−1 ± 0.1 (10−1)
sir3-A2T7 × 10−1 ± 0.6 (1)4 × 10−4 ± 4 (10−3)4 × 10−2 ± 2 (10−1)
sir3-R30K8 × 10−1 ± 0 (1)2 × 10−5 ± 0.9 (10−5)4 × 10−2 ± 2 (10−1)
sir3-R92K9 × 10−1 ± 0.8 (1)1 × 10−5 ± 0.7 (10−5)2 × 10−2 ± 0.6 (10−2)
sir3-L96F9 × 10−1 ± 0.4 (1)3 × 10−5 ± 0.4 (10−5)4 × 10−2 ± 0 (10−1)
sir3-8(E131K)7 × 10−1 ± 0.2 (1)4 × 10−3 ± 4 (10−2)2 × 10−1 ± 0.5 (10−1)
sir3-T135I5 × 10−1 ± 0.3 (1)<2 × 10−6 ± 0.5 (10−6)3 × 10−3 ± 0.3 (10−3)
sir3-E140K7 × 10−1 ± 0.7 (1)4 × 10−6 ± 0.4 (10−5)2 × 10−3 ± 0.8 (10−3)
sir3-L208S3 × 10−1 ± 0.8 (10−1)<3 × 10−6 ± 1 (10−6)3 × 10−3 ± 2 (10−3)
sir3-S813F1 × 10−1 ± 0.2 (10−1)7 × 10−6 ± 3 (10−5)3 × 10−2 ± 0.9 (10−2)
Vector only1 × 10−5 ± 0.4 (10−5)4 × 10−6 ± 1 (10−5)2 × 10−1 ± 0 (10−1)
  • a Mating efficiency is expressed as a mean of two experimental values, with the range indicated. In parentheses, each efficiency is presented relative to this wild-type plasmid control, rounded to the nearest exponent.

  • b Plasmids used were pLP187, pLP189, pLP1131, pLP1048, pLP464, pLP675, pLP946, pLP469, pLP473, pLP472, and pRS313, in descending order.