TABLE 1

Yeast strains

StrainGenotypeSource
W303-1aMATa ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100R. Rothstein
W303-1bW303-1a MATαR. Rothstein
AMR7W303-1a nat1-3::URA3Stone et al. (1991)
AMR27W303-1a sir1::LEU2Stone et al. (1991)
RS862W303-1a sir3::TRP1Stone et al. (1991)
YDS631W303-1b adh4::URA3-(C1-3A)nChien et al. (1993)
YDS634W303-1b adh4::URA3-4xUASG-(C13A)nChien et al. (1993)
JRY188MATα his4am leu2 rme1 sir3-8 trp1am ura3-52J. Rine
JRY3010AMR27 MATαJ. Rine
JRY4603W303-1b sir3::HIS3 ADE2 lys2J. Rine
JRY4623W303-1b sir1::TRP1 ADE2 lys2J. Rine
381GMATa SUP4-3 cry1 his4-580 trp1 ade2-1 tyr1 lys2Hartwell (1980)
EY957W303-1a bar1E. Elion
LPY78MATα his4P. Schatz
LPY142MATa his4P. Schatz
LPY221AMR27 sir3-R30KThis study
LPY222AMR27 sir3-T135IThis study
LPY225AMR27 sir3-E140KThis study
LPY238AMR27 sir3-R92KThis study
LPY275AMR27 sir3-L96FThis study
LPY521AMR27 sir3-E140KThis study
LPY669AMR27 sir3-S813FThis study
LPY683AMR27 sir3-L208SThis study
LPY1132W303-1b nat1-3::URA3 sir3::TRP1This study
LPY2709EY957 hmlΔ::TRP1 sir3::HIS3This study
LPY3237W303-1b sir1::LEU2 adh4::URA3-(C1-3A)nThis study
LPY3238W303-1b sir3::TRP1 adh4::URA3-(C1-3A)nThis study
LPY3320W303-1a sir1::TRP1 sir3::TRP1 adh4::URA3-(C1-3A)nThis study
LPY3321W303-1b sir1::TRP1 sir3::TRP1 adh4::URA3-(C1-3A)nThis study
LPY3620W303-1a sir3::TRP1 adh4::URA3-(C1-3A)nThis study
LPY4417W303-1b adh4::URA3-4xUASG-(C1-3A) sir3::TRP1This study
LPY4441W303-1a hmlΔ::TRP1This study