TABLE 1

Microconidia production by the ami1-1 mutant and other relevant strains

StrainsaNo. of microconidiabNo. of peritheciab
5 daysc20 days30 days4 days15 days
Wild type≤1045 × 1062 × 10784 ± 5 (102)28 ± 3 (104)
incA6 × 1078 × 1078 × 107104 ± 10 (105)154 ± 11 (105)
ami1-1≤1044 × 1055 × 1061.5 ± 0.5d11 ± 5d
ami1-1 incA≤1041.5 × 1062 × 1060d50 ± 4d
ami1-1 (apsA-1)≤1043 × 1052 × 10652 ± 5 (101)54 ± 9 (102)
ami1-1 (apsA-12)≤104ND10612 ± 0.5d168 ± 10d
  • ND, not determined.

  • a (apsA-1) and (apsA-12) correspond to two integration events of the A. nidulans apsA gene.

  • b Microconidia were recovered at different times (from 5 to 30 days) by washing the surface (~60 cm2) of one petri dish of each culture. They were counted using a hemacytometer chamber. The numbers correspond to 1 ml of suspension. Perithecia were counted after fertilization of the wild-type strain used as the female partner with 1 ml of microconidia suspension of the relevant strain. The numbers are the mean values of three to four petri dishes with, in parentheses, the dilution factor of the microconidia suspension (standard deviation).

  • c Values in the range of 104 (and less) cannot be reliable since they correspond to the lowest limit of the chamber. Otherwise, we did not have confidence in concentrated microconidia suspensions obtained by centrifugation because they gave variable results from one strain to the other: in particular, it was observed that in strains carrying the ami1-1 mutation, the pellets contained highly enriched populations of nucleate microconidia.

  • d Undiluted.