TABLE 2

Protein interactions of Mre11(ts)

Ga14AD fusions
LexA fusionspGAD424HomodimerizationRad50Xrs2
pBTM116a
Mre11++++++++/−
Mre11 1-559+++++++++
Mre11(ts)
Mre11(ts) 1-559+
  • The permissive temperature was 24°. No interactions were observed at 34° for Mre11(ts).

  • a β-Gal activities were determined by a filter assay as described in materials and methods. Color development was scored as follows: +++++, <15 min; ++++, 15 min–1 hr; +++, 1–4 hr; ++, 4–8 hr; +, 8–12 hr; +/−, 12–24 hr. Colonies remaining white after 24 hr were considered negative.