TABLE 5

Summary of genetic interactions between glc8, glc7, ppz1, and ppz2

Relevant genotypeTetrads analyzedNo. of spores of relevant genotypeaGrowth rate of relevant genotypebViability of other spore clones (%)
ppz1 ppz2 glc8115Wild type (5/5)39/39 (100)
glc7-109 glc81211Slow growth (11/11)36/37 (97.3)
glc7-127 glc8117Slow growth (7/7)32/37 (86.5)
glc7-129 glc8119Inviable (9/9)33/35 (94.3)
glc7-132 glc81112glc7-132c (12/12)32/32 (100)
glc7-133 glc81213Very slow growth (13/13)32/35 (91.4)
  • a Where the spore clones did not grow into macrocolonies, the genotype of the mutants was inferred from the genotypes of viable spore clones in the tetrad, assuming no gene conversion.

  • b Growth rate was assessed by colony size of spore clones.

  • c Growth rate was comparable to the glc7 mutant alone.