TABLE 3

Summary of glc7 ppz1 ppz2 triple-mutant phenotypes

glc7 alleleglc7 mutationglc7aglc7 ppz1 ppz2a,b
glc7-109K259A, R260ASalt sensitive, high glycogen, caffeineSHigh glycogen, slg, YPGE, caffeineS, ts, cs
glc7-127K110A, K112Aslg, low glycogen, weakly salt sensitive, 2-DGr, spoInviable
glc7-129D137A, E138Aslg, cold-sensitive mitotic defect, low glycogen, spoInviable
glc7-132D165A, E166A, K167Aslg, low glycogen, weakly salt sensitiveLow glycogen, slg, cs, caffeineS
glc7-133R186A, R187A, R190A, Q298A2-DGrVery slow growth, cell lysis defect
  • a The defects are reduced sporulation (spo), slow growth at 24–30° (slg), temperature sensitivity (ts), cold sensitivity (cs), 2-deoxyglucose resistance (2-DGr), and failure to utilize glycerol and ethanol (YPGE).

  • b With the exception of salt sensitivity, glc7 traits were not affected in PPZ1 PPZ2, ppz1::URA3 PPZ2, or PPZ1 ppz2::LEU2 backgrounds.