TABLE 3

uvsC induction in sepB3 and sepB3 uvsB110 mutants

StrainGenotypeuvsC induction ratio
ASH208sepB32.3 ± 0.6
ASH202sepB3 uvsB1101.1 ± 0.1
  • Strains were grown at either permissive (28°) or restrictive temperature (42°). uvsC transcript levels were determined by Northern analysis and quantification of band intensities using a phosphoimager. Transcript levels were normalized to the loading control (actin) by dividing the counts per minute in the uvsC bands by the counts per minute in the actin bands. Induction ratios represent the ratio of uvsC transcript levels at 28° compared to 42°. The data shown represent the mean average of two independent experiments.