TABLE 2

Effects of the uvsB110 and musN227 mutations on DNA damage-induced transcription

StrainGenotypeuvsC induction ratio
A28Wild type3.3 ± 0.4
ASH201uvsB1102.1 ± 0.1
ASH383musN2272.7 ± 0.4
AAS315uvsB110 musN2272.8 ± 0.1
  • Transcript levels were determined by Northern analysis and quantification of band intensities using a phosphoimager. Levels were normalized to the loading control (actin) by dividing the counts per minute in the uvsC bands by the counts per minute in the actin bands. Induction ratios represent the ratio of treated levels to untreated levels. The data shown represent the mean average of two independent experiments.