Basal cAMP levels in S. pombe strains

StrainRelevant genotypeBasal cAMPGlucose-induced cAMP
Experiment 1a
FWP94gpa2+git5+3.3 ± 0.5ND
CHP463git5Δ3.2 ± 0.6ND
CHP462gpa2Δ2.0 ± 0.2ND
CHP469gpa2Δ git5Δ1.9 ± 0.4ND
Experiment 2b
FWP94gpa2+git5+3.0 ± 0.711.2 ± 3.9
CHP463git5Δ4.3 ± 0.84.7 ± 0.9
  • a From five to nine independent cultures of each strain were grown to logarithmic phase under low glucose conditions (0.1% glucose, 3% glycerol) and assayed as previously described (Byrne and Hoffman 1993) using a RIA assay kit (Amersham, Piscataway, NJ).

  • b Three independent cultures of each strain were grown as in experiment 1. cAMP levels were determined from cells collected prior to, and 1 min after, the addition of glucose to a final concentration of 100 mm. Values indicate mean ± standard error of the pmol cAMP/mg total protein.