TABLE 2

β-Galactosidase activity from fbp1-lacZ reporter

Plasmid-expressed gene
Host genotypeEmpty vectorgpa2git5agit5b
git+881524
gpa2Δ20791319051802
git5Δ9221801539
  • Strains FWP112 (git+), CHP439 (gpa2Δ), and CHP477 (git5Δ) were transformed to Leu+ with pART1 (empty vector; McLeod et al. 1987), pGMSK1 (gpa2; Nocero et al. 1994), pMP1 (git5a; genomic git5 clone), or pSL11 (git5b; SspI sub-clone of git5 into pART1). Two independent transformants from each transformation were purified and grown to exponential phase under glucose-rich (8%) conditions. β-Galactosidase expressed from the fbp1-lacZ fusion was determined from two to four independent cultures of each transformant as described previously (Nocero et al. 1994). Standard errors were <20% of the mean.