TABLE 1

S. pombe strains used in this study

StrainGenotypeSource
rad2Δh rad2::ura4+ ura4 D18 leul-32 ade6704A. M. Carr
IBGY5-1h smt-0 ura4-D18 leul-32V. I. Bashkirov
IBGY10-1h+ mat1PΔ17::LEU2 ura4-D18 leu1-32 his3-D1 arg61V. I. Bashkirov
TMPR2Cah rad2::ura4 ura4 D18 leu1-32 ade6-704/pAUR2bThis study
TMPR2-2 (slr2)h+ rhp57-1 ura4-D18 leu1-32This study
TMP701ch+ ura4-D18 leu1-32 his7-366 ade6-M216This study
TMP702h+ ura4-D18 leu1-32This study
TMP703h ura4-D18 leu1-32This study
TMP711 (rhp57Δ)h+ rhp57::ura4+ ura4-D18 leu1-32 his7-366 ade6-M216This study
TMP712 (rhp51Δ)h+ rhp51::ura4+ ura4-D18 leu1-32 his7-366 ade6-M216This study
TMP713h+ rhp51::ura4+ rhp57::LEU2 ura4 D18 leu132 his7-366 ade6M216This study
(rhp51Δ rhp57Δ)
TMP714h+ rhp57::LEU2 ura4-D18 leu1-32 his7-366 ade6-M216This study
TMP715h rhp57::LEU2 ura4-D18 leu1-32 his7-366This study
TMP721h+ rad2::ura4+ ura4-D18 leu1-32This study
TMP751h+ mat1PΔ17::LEU2 ura4-D18 leu1-32 ade6-M216This study
TMP752h+ mat1PΔ17::LEU2 rhp57::ura4+ ura4-D18 leu1-32 ade6-M216This study
TMP753h smt-0 rhp57::ura4+ ura4-D18 leu1-32This study
  • a The rad2::ura4 strain was isolated from the rad2Δ cells by selecting a colony formed on an EMM plate containing 5-FOA, uracil, leucine, and adenine. The UV sensitivity of the resulting Ura colony was the same as that of the parental rad2Δ strain.

  • b The plasmid pAUR2 carries the S. pombe ura4 and rad2 genes and the arsl sequence.

  • c TMP701 was used as a wild-type haploid strain for MMS and λ-ray sensitivity tests and for homologous integration assays.