TABLE 2

Sequences of the synthetic oligonucleotides used for PCR amplification

GeneForward primerReverse primer
mde15′-GGGGTCGAC(Sa1I)CGTTGAATGATAGCTCTATAG-3′5′-CCCGTCGAC(SalI)TATCATCTTCCTCCCTCTTCC-3′
mde25′-C AAGGGGTGCGAAGG-3′5′-CTCAGCTGACGCAGG-3′
mde35′-CCCGTCGAC(SalI)ATGAGTAATGAAAGTATTTACTCAGTG-3′5′-CCCGGATCC(BamHI)TGGCGAACTTAGATGTAAGC-3′
mde45′-CCCCTCGAG(XhoI) GATGCTAAAGGTGGGCGATC-3′5′-CCCGCGGCCGC (NotI) GTAACACGATCAGGATGTGC-3′
mde55′-GGTGGTAATCCCGGC-3′5′-CATGGATGTTATTCAACGC-3′
mde65′-ACGCATCAGAGGCGC-3′5′-CAGCCAACTGTTCCC-3′
mde75′-CCCCTCGAG(XhoI)GGAGCATTTAATAAACCCCC-3′5′-CCCGCGGCCGC(NotI) GAAAGCAACAGCCTTCGCTCG-3′
mde85′-CCCCTCCAGATACAG-3′5′-CCCAAGACATCGTCC-3′
mde95′-CAGGCCCGATTTCC-3′5′-CGCAGGACGGCCAGC-3′
rec65′-CGTCTCGGTACATCATAAAGAGCTC-3′5′-CCTTACTCAGTCTGCAGCCGAAAGC-3′
recl25′-CTAATTGCTTGTAACAGGCTAAAGG-3′5′-TCCAAGAACTTAACGTACTGAGTGC-3′