TABLE 3

Southern blot and DNA-mediated transformation analyses identify shared alleles in nine different B specificities of C. cinereus

Host strainsa
B42B6B40B41B3B1B44B5B43
GenesH/CH/CH/CH/CH/CH/CH/CH/CH/C
Group 142+/−+/−−/+−/+−/++/−+/−+/−−/+
Group 16+/−+/−−/+−/+−/++/−+/−+/−−/+
Group 13−/+−/++/−+/−+/−−/+−/+−/++/ −
Group 242+/−−/+−/+−/++/−−/+−/+−/+−/+
Group 26−/++/−−/+−/ +−/+−/+−/+−/+−/+
Group 342+/−−/+−/+−/+−/+−/+−/+−/+−/+
Group 36−/++/−+/−+/−−/+−/+−/+−/+−/+
Group 33−/+−/+−/+−/++/−+/−+/−−/+−/+
  • a B42 (LN118), B6 (LT2), B40 (LCO7), B41 (LCO5), B1 (218), B44 (NL1), B5 (HT8), B43 (AT8). H, Southern analysis; C, B-regulated development in transformed host. +, DNA hybridization or activation of B pathway; −, absence of either hybridization or B-regulated development. Underlined entries indicate homoallelism for genes of a given group between different specificities. These data are summarized in Figure 2. Hybridization data were identical for each of the three genes present in each B42 and B6 group. Scores for transformation with B42 and B6 genes represent the activity of the receptor gene and at least one of the two pheromone genes within each group.